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. Author manuscript; available in PMC: 2013 Jul 15.
Published in final edited form as: Nat Chem Biol. 2012 Oct;8(10):839–847. doi: 10.1038/nchembio.1060

Figure 6. PKM2 activators impair xenograft growth.

Figure 6

(a) Mice bearing H1299 xenograft tumors received bolus doses of TEPP-46 at 50 mg/kg 16 hours and 4 hours before sacrifice. Tumors were dissected and PKM2 complex stoichiometry in tumor lysates was determined by size exclusion chromatography. Uncropped blots are shown in Supplementary Fig. 10. (b) Concentrations of lactate, ribose phosphate and serine in H1299 xenograft tumors from mice treated with vehicle or TEPP-46 as in (a). (c) H1299 cells were injected subcutaneously into nu/nu mice which were subsequently randomly divided into two cohorts, one given vehicle and the other TEPP-46 at 50 mg/kg twice-daily throughout the duration of the experiment. Injection sites were monitored for tumor emergence [p value calculated by logrank (Mantel-Cox) test]. After 52 days, the tumors were dissected and final tumor weights were measured (d). Mean tumor weights ± s.e.m. are shown and p value was calculated by unpaired Student’s t-test.