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. Author manuscript; available in PMC: 2013 Jul 15.
Published in final edited form as: Cell Rep. 2013 Apr 25;3(5):1690–1702. doi: 10.1016/j.celrep.2013.03.039

Figure 1. Identification of T3SS2-Secreted Polypeptides.

Figure 1

(A) DIGE of supernatant-derived proteins from a T3SS1-deficient strain (RIMD2210633 vscN1; red) and a T3SS1/T3SS2-deficient strain (RIMD2210633 vscN1 vscN2; green). Spots selected for MALDI tandem mass spectrometry are circled, and protein identities and functions are indicated below.

(B) Schematic depiction of the T3SS2 region from V. parahaemolyticus RIMD2210633, including most genes from vpa1321 to vpa1370. Genes encoding effectors are colored red; genes encoding structural components are colored gray. The transcriptional regulator vtrB (vpa1348) is colored black.

(C) Identity of the spots identified from DIGE and mass spectrometry. Black print shows proteins encoded in T3SS2, gray print indicates proteins encoded outside of T3SS2, and red type indicates effector proteins. Final column shows whether T3SS2-dependent secretion has been confirmed: yes (Y), no (N), not determined (ND). Asterisk indicates previously unidentified T3SS2-secreted proteins.