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. Author manuscript; available in PMC: 2013 Jul 15.
Published in final edited form as: Immunity. 2007 Oct 11;27(4):561–571. doi: 10.1016/j.immuni.2007.09.005

Figure 3.

Figure 3

Pro-B cell lysates contain RAG-2 bound to histone H3 di- or trimethylated at lysine 4. Bone marrow cells were cultured in IL-7 for 10 d to generate B220+CD43+ pro-B cells. Protein was immunoprecipitated from pro-B cell lysates with an antibody specific for H3K4me2 and H3K4me3 or with an isotype control. Immunoprecipitates (lanes 2 and 3) were fractionated by SDS-PAGE and assayed by immunoblotting for RAG-2 (top), H3K4me3 (middle) and histone H3 (bottom). Input protein (20%) was assayed as a reference (lane 1).