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. 2012 Apr 18;53(4):2030–2037. doi: 10.1167/iovs.11-8430

>Figure 1.

>Figure 1.

Effect of varying concentrations of NGF on proliferation of (A) CD34+ cells and (B) HRECs during a 72-hour period. Proliferation of CD34 cells was examined using the trypan blue–exclusion method and HREC proliferation was examined using the MTT assay. MTT activity was expressed in arbitrary fluorescence units. Data presented as mean ± SE (n = 6). Data analysis: two-way ANOVA revealed significant effects of concentration of NGF (F[3,60] = 24.97; P < 0.0001), duration of incubation with NGF (F[2,60] = 9.38; P = 0.0003), and interaction of concentration and incubation period (F[6,60] = 23.91; P < 0.0001) on proliferation of CD34+ cells; each of these factors accounted for 25.21%, 6.32%, and 48.28% of the total variance, respectively. Asterisks indicate significant difference compared with corresponding control (post hoc Bonferroni multiple comparisons).