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. Author manuscript; available in PMC: 2014 Jun 1.

Published in final edited form as: Breast Cancer Res Treat. 2013 May 18;139(2):351–360. doi: 10.1007/s10549-013-2541-y

Fulvestrant induced EGFR family member activation was ER dependent. MCF-7L and C4-12 (an ER negative cell line derived from MCF-7L cells) were starved in SFM for 24 hours, then cells were treated with (Fulv) or without (SFM) 100 nM fulvestrant for 48 hours. (A) 50 μg cell lysates were used for immunoblotting of ERα and receptor phosphorylation by using anti phosphotyrosine (PY20) antibody. Actin blot was used as loading control. (B) C4-12 cells were used for HB-EGF and TGFα mRNA quantification. % change of mRNA levels was shown in the figure by using SFM as control.

Fulvestrant induced EGFR family member activation was ER dependent. MCF-7L and C4-12 (an ER negative cell line derived from MCF-7L cells) were starved in SFM for 24 hours, then cells were treated with (Fulv) or without (SFM) 100 nM fulvestrant for 48 hours. (A) 50 μg cell lysates were used for immunoblotting of ERα and receptor phosphorylation by using anti phosphotyrosine (PY20) antibody. Actin blot was used as loading control. (B) C4-12 cells were used for HB-EGF and TGFα mRNA quantification. % change of mRNA levels was shown in the figure by using SFM as control.