Skip to main content
. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Eur J Neurosci. 2012 Oct 8;37(2):242–250. doi: 10.1111/ejn.12010

Fig. 4.

Fig. 4

Knockdown of mPer1 and mPer2 in the NAc increases anxiety. Mice were infused with the AAV-mPer1;mPer2 shRNA (sh) or AAV-scrambled control shRNA (Scr) into the NAc and behavior was measured 2 weeks later. (A) Representative image showing viral expression in the NAc and DAPI staining of the same slice. Scale bar, 500 µm. (B) AAV-mPer1;mPer2 infusion leads to a significant knockdown of mPer1 and mPer2 expression in the NAc (n = 6, Scr-infected mice; n = 5–6 mPer1;mPer2 shRNA-infected mice with two biological replicates of each). (C)AAV-mPer1;mPer2 shRNA NAc-infected mice (n = 10) had fewer open arm entries in the EPM than controls (n = 11) (P < 0.05, t-test). (D) AAV-mPer1;mPer2 shRNA NAc-infected mice spent less time in the center of the open field (*P<0.05, t-test).