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. 2013 Feb 13;33(7):3113–3130. doi: 10.1523/JNEUROSCI.3467-12.2013

Figure 3.

Figure 3.

Apc deletion reduces OPC proliferation. Tamoxifen was injected in pups at P6 and P7 and CNS tissues were analyzed as indicated. A1–A5, Confocal images of PH3/Ki67 and PH3/SOX10/NG2 immunostaining and quantifications of cells positive for these markers in Apcfl/fl WT and OCE/Apcfl/fl KO (n = 3 WT, 3 KO at P8; n = 10 WT, 7 KO at P14). B1, B2, Representative confocal images of EdU (2 h labeling). C, The number of Sox10+/NG2+ OPCs in the spinal WM and GM of P14 Apcfl/fl WT (n = 10) and OCE/Apcfl/fl KO mice (n = 7). D, Western blot of cyclin D1 in P14 spinal cord. E, Quantification of cyclin D1 protein (left) and mRNA (right) levels in P14 Apcfl/fl WT (n = 4) and OCE/Apcfl/fl KO (n = 4) mice. Scale bars: A1–A4, 10 μm; B1, B2, 50 μm.