Fig 2.

Binding and transport activity of HSC channel in reconstituted proteoliposomes. a, Measurements of sulfide concentrations in the media of salmonella transformed with vector control or vector encoding FNT3. Minimal media was supplemented with either sulfite or thiosulfate to induce hydrogen sulfide production from either periplasmic thiosulfate reductase or cytoplasmic sulfite reductase. b, Binding of detergent solubilized and purified HSC protein to various anions was determined by using thermostability coupled size exclusion chromatography. Peak heights of recovered samples were plotted against temperature and fitted to a boltzman-sigmoidal model to determine nominal melting temperatures. c, Radiolabeled formate uptake in proteoliposomes reconstituted with purified FNT at pH 8.0 was monitored in a concentrative uptake assay and compared to FocA activity or vesicle controls. d, Inhibition of radiolabeled concentrative uptake of formate by the addition of various anions at increasing concentrations. The plotted bar graphs represent the amount of radiolabeled formate measured at the 10-minute time point for each concentration of anion tested. The concentration of the competing anions were, 0 mM, 0.15 mM, 0.6 mM, 3 mM and 15 mM. Error bars represent s.e.m. (N = 3).