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. Author manuscript; available in PMC: 2013 Jul 15.
Published in final edited form as: Nat Biotechnol. 2013 Apr 21;31(5):440–447. doi: 10.1038/nbt.2565

Figure 1.

Figure 1

SHH-dependent specification of basal forebrain progenitors from hESCs. (a) Schematic showing specification of NKX2.1+ ventral progenitors (VP) from neuroepithelia (NE) by SHH for 2 weeks starting from day 10. (b) Quantitative RT-PCR analysis of various markers (expression relative to control: 0 ng ml−1 SHH treatment group) on indicated days of differentiation. Dorsal and ventral refer to dorsal and ventral forebrain markers. Error bars, s.e.m. (c) Immunocytochemistry analysis of hESC-derived neural progenitors for NKX2.1 and PAX6 expression in response to SHH on day 25 of differentiation. Scale bar, 50 μm. (d) Quantification of data in c. Error bars, s.e.m.; *P < 0.05 and **P < 0.01. (e) Western blot analysis of hESC-derived neural progenitor lysates with antibodies to indicated proteins in response to SHH treatment on day 25 after differentiation.