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. Author manuscript; available in PMC: 2013 Jul 15.
Published in final edited form as: Nat Biotechnol. 2013 Apr 21;31(5):440–447. doi: 10.1038/nbt.2565

Figure 2.

Figure 2

Differentiation of basal forebrain progenitors to cholinergic neurons and GABAergic interneurons. (a) Schematic of neuronal differentiation from basal forebrain progenitors. NE, neuroepithelia. (b) Quantification of the percentage of ChAT+ and GABA+ neurons in βIII-tubulin+ (βIII-T+) cells. Error bars, s.e.m. (c) Immunofluorescence images of ChAT+ cholinergic neurons immunostained for βIII-tubulin (βIII-T), VAChT, NKX2.1 and FOXG1. (d) Immunofluorescence images of GABA neurons immunostained for βIII-tubulin (βIII-T), NKX2.1 and GAD65. TO, topo3; HO, Hoechst staining. Scale bars, 50 μm.