Post-thaw (a) total sperm motility (% tMOT) and (b) acrosome intact status (% INT) of Asian elephant spermatozoa treated with different concentrations of cholesterol-loaded cyclodextrin (CLC; 0, 0.5, 1.5, 3.0, 4.5, 6.0 and 7.5 mg CLC per 120 × 106 spermatozoa mL–1) before cryopreservation. Values are the mean±s.e.m. The dotted line represents the mean motility of the raw ejaculate before CLC exposure and cryopreservation. Within each time point, CLC treatments with different letters differ significantly (P < 0.05).