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Comparison of post-thaw (a) total sperm motility (% tMOT) and (b) acrosome intact status (% INT) of Asian elephant spermatozoa exposed to glycerol at either 22°C or 4°C before cryopreservation. Values are the mean±s.e.m. The dotted line represents the mean motility of the raw ejaculate before CLC exposure and cryopreservation. Within each time point, bars with asterisks differ significantly (P < 0.05).
