Table 2.

Comparison of post-thaw progressive sperm motility of spermatozoa treated with different concentrations of cholesterol-loaded cyclodextrin and exposed to glycerol addition at either 22°C or 4°C before cryopreservation

Glycerol addition	Time	CLC (mg per 120 × 106 spermatozoa mL–1)
		0	0.5	1.5	3.0	4.5	6.0	7.5
22°C	Post-thaw	8.3 ± 4.6b	20.0 ± 7.5a2	21.1 ± 8.8a2	8.9 ± 5.0a2	6.1 ± 3.9b	3.3 ± 1.7b	1.7 ± 1.2b
	Dilution 0 h	1.9 ± 0.8c	12.1 ± 3.5b2	20.6 ± 4.0a2	3.9 ± 1.4c2	1.7 ± 1.1c	0.6 ± 0.4c	0.6 ± 0.6c
	Dilution 1 h	1.1 ± 0.6b2	10.0 ± 2.9a2	15.0 ± 3.3a2	2.2 ± 1.2b2	0.8 ± 0.6b	0.3 ± 0.2b	0.3 ± 0.3b
	Dilution 2 h	0 ± 0b2	8.1 ± 2.4a2	9.4 ± 3.2a2	1.4 ± 1.1b2	0.7 ± 0.6b	0.2 ± 0.1b	0.3 ± 0.3b
	Dilution 3 h	0 ± 0b	6.4 ± 2.5a2	7.2 ± 2.6a2	0.8 ± 0.8b2	0.3 ± 0.3b	0.1 ± 0.0b	0.0 ± 0.0b
	Dilution 4 h	0 ± 0b	5.3 ± 2.3a2	6.9 ± 2.5a2	0.8 ± 0.8b	0.3 ± 0.3b	0.0 ± 0.0b	0.0 ± 0.0b
4°C	Post-thaw	16.1 ± 6.6c	35.0 ± 5.5ab1	45.0 ± 6.0a1	28.9 ± 4.1bc1	15.6 ± 3.7c	10.6 ± 2.4c	9.2 ± 1.4c
	Dilution 0 h	12.4 ± 4.7cd	29.4 ± 3.9b1	38.1 ± 3.5a1	14.7 ± 2.1c1	5.8 ± 2.1de	3.8 ± 0.9e	1.3 ± 0.6e
	Dilution 1 h	9.3 ± 3.7b1	22.2 ± 4.4a1	28.6 ± 3.7a1	9.0 ± 2.1b1	3.3 ± 1.3bc	1.3 ± 0.6c	0.6 ± 0.4c
	Dilution 2 h	6.9 ± 2.9c1	17.5 ± 3.7b1	23.1 ± 3.4a1	7.2 ± 1.6c1	1.8 ± 1.0cd	0.4 ± 0.3d	0.3 ± 0.3d
	Dilution 3 h	4.7 ± 2.0cd	15.6 ± 3.5b1	20.8 ± 3.3a1	6.3 ± 1.5c1	1.1 ± 0.8de	0.0 ± 0.0e	0.0 ± 0.0e
	Dilution 4 h	3.2 ± 1.8b	10.6 ± 2.7a1	14.4 ± 3.1a1	4.0 ± 1.4b	0.6 ± 0.6b	0.0 ± 0.0b	0.0 ± 0.0b

Values are the mean ± s.e.m. Values with different superscript letters among cholesterol-loaded cyclodextrin (CLC) treatments within the same time point and glycerol treatment differ significantly (P < 0.05). Values with different superscript numbers between glycerol addition temperatures, within the same time point and CLC level, differ significantly (P < 0.05). % pMOT, progressive sperm motility