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. Author manuscript; available in PMC: 2014 Jul 16.
Published in final edited form as: Oncogene. 2013 Jan 14;33(3):358–368. doi: 10.1038/onc.2012.582

Figure 1.

Figure 1

EcSOD expression is detectable in normal breast tissue but not in non-malignant mammary epithelial cells in monolayer cultures. (a) Total RNAs were extracted and analyzed for EcSOD mRNA transcription from post-stasis HMEC, which were derived from reduction mammoplasties and a spontaneously transformed non-malignant mammary epithelial cell line, MCF-10A. In comparison with normal human lung fibroblasts (MRC5) and normal human airway epithelial cells, HMEC and MCF-10A cells showed undetectable EcSOD mRNA expression as determined by a real-time RT–PCR analysis. 18S was used as a loading control. Error bars represent s.e.m., n = three separate experiments. (b) IHC analysis demonstrates a positive staining for EcSOD in normal mammary tissue (right panel). Left panel shows H and E staining of the same normal mammary tissue.