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. Author manuscript; available in PMC: 2014 Jul 16.
Published in final edited form as: Oncogene. 2013 Jan 14;33(3):358–368. doi: 10.1038/onc.2012.582

Figure 5.

Figure 5

Overexpression of EcSOD inhibited stellate colony formation of MDA-MB231 cells in 3D culture. Two-dimensional cultured MDA-MB231 were infected with AdEmpty, AdEcSOD or AdΔHBD, at moi = 50 PFU/cell for 24 h, followed by reseeding the cells in 3D culture. After 5 days of 3D culture, MDA-MB231 cells formed large colonies with extensive stellate structures, which is a hallmark feature of the ‘invasive’ breast cancer 3D phenotype. While AdEmpty did not affect the 3D morphology of this cell line, overexpression of EcSOD, both the full-length and the truncated forms (with AdEcSOD and AdΔHBD adenovirus infection, moi = 50 PFU/cell) resulted in much smaller colonies which lack stellate formation. A similar 3D morphological alteration was observed when MDA-MB231 cells were generated to stably over express EcSOD, in Ec.20 (full length) and ΔHBD.3 (deletion in the HBD) where the cells formed small colonies with an absence of stellate extension. Representative micrographs of N = 3. Bar scale = 100 μm.