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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: Cell Microbiol. 2013 Mar 14;15(8):1401–1418. doi: 10.1111/cmi.12125

Figure 1. Immunofluorescence microscopy showing staggered delivery of different exomembrane components.

Figure 1

(A-D) Infected RBCs were synchronized to a 1 h window, samples were collected at 2 h intervals and smears were fixed with acetone and stained with antibodies recognizing REX1, MAHRP1, SBP1, MAHRP2 and PfEMP1 (ATS) and co-stained with DAPI. Images are presented at time points before and after delivery to the RBC cytoplasm. Scale bars = 3 μm. (E) Mean percentages ± S.E. of Maurer’s clefts (as determined by REX1 labeling) that have detectable levels of SBP1, MAHRP1 and MAHRP2. (Based on analysis of 5-10 cells).