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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: Cell Microbiol. 2013 Mar 14;15(8):1401–1418. doi: 10.1111/cmi.12125

Figure 3. Live cell time course showing assembly of exomembrane components.

Figure 3

Transfectants expressing GFP chimeras of (A, B) REX1 and (C, D) MAHRP2 were co-labeled with BODIPY-ceramide. Infected RBCs were synchronized to a ~2 h window and samples were collected at 4 h intervals. BODIPY-ceramide- and REX1-GFP-labeled Maurer’s clefts and MAHRP2-GFP-labled structures are evident in the RBC cytoplasm from ~4 h post invasion. MAHRP2-GFP-labled structures are associated with the BODIPY-ceramide-labeled Maurer’s clefts (D, white arrows). (E) Quantitative analysis of the number of REX-GFP and MAHRP2-GFP labeled structures in the RBC cytoplasm at different time points. (F) MAHRP2-GFP transfectants were permeabilized with EqtII and labeled with antibodies recognizing REX1. Scale bars = 3 μm (A, C), 1 μm (B, D) and 4 μm (F). See Figure S1, S2 for time series.