Table 1. Primer sequences and thermal conditions of PCR analysis.

Gene	Oligonucteotide sequence (5′-3′)	Temperature of annealing	Size of PCR product (bp)	Reference
Pta	AAA GAC AAA CTT TCA GGT AA GCA TAA ACA AGC ATT GTA CCG	53°C	320	Bannoehr et al.⁷
Sea	CCT TTG GAA ACG GTT AAA ACG TCT GAA CCT TCC CAT CAA AAA C	55°C	127	Becker et al.¹⁵
Seb	TCG CAT CAA ACT GAC AAA CG GCA GGT ACT CTA TAA GTG CCT GC	55°C	477	Becker et al.¹⁵
Sec	CTC AAG AAC TAG ACA TAA AAG CTA GG TCA AAA TCG GAT TAA CAT TAT CC	55°C	271	Becker et al.¹⁵
Sed	CTA GTT TGG TAA TAT CTC CTT TAA ACG TTA ATG CTA TAT CTT ATA GGG TAA ACA TC	55°C	319	Becker et al.¹⁵
See	CAG TAC CTA TAG ATA AAG TTA AAA CAA GC TAA CTT ACC GTG GAC CCT TC	55°C	178	Becker et al.¹⁵
Tst	AAG CCC TTT GTT GCT TGC G ATC GAA CTT TGG CCC ATA CTT T	55°C	445	Becker et al.¹⁵
Siet	ATG GAA AAT TTA GCG GCA TCT GG CCA TTA CTT TTC GCT TGT TGT GC	56°C	359	Lautz et al.¹⁴
lukS	TGT AAG CAG CAG AAA ATG GGG GCC CGA TAG GAC TTC TTA CAA	57°C	503	Futagawa-Saito et al.¹³
luk F	CCT GTC TAT GCC GCT AAT CAA AGG TCA TGG AAG CTA TCT CGA	57°C	572	Futagawa-Saito et al.¹³