Figure 6. Effect of SAHA and tubastatin A on the activity of Src family kinases. JURL-MK1 cells were treated with 0.5 µM SAHA or 10 µM tubastatin A for up to 24 h and the level of autophosphorylated (active) Src kinases as well as that of Lyn kinase were assessed by western-blotting. (A) Representative blots from SAHA-treated cells at different incubation times. The effect of 30 min treatment with 100 nM TPA is shown in the last lane for comparison. (B) Summary results of anti-pSFK western-blots from repeated experiments (n = 4 for SAHA, n = 3 for tubA). The sum of the two main pSFK bands from each lane was expressed as relative to the sum in the corresponding control lane.