Figure 1. RhoGDIα mRNA expression in MCF7 and MDA-MB-231 cells. (A) Relative RhoGDIα mRNA levels between untreated MCF7 and MAD-MB-231 cells with or without E2 were not significantly different. RhoGDIα expression was decreased at 24, 48, and 72 h after transfection with siRNA. Values represent averages of three independent experiments with error bars indicating the standard deviation. (B) The total mRNA levels in untreated cells were set as 100%. mRNA expression levels showed 90% decrease at 24 and 48 h after siRNA treatment in both cell lines.