Figure 3. (A) Representative photographs of MCF7 and MDA-MB-231 cells invasion assays. Downregulation (a) and upregulation (b) of RhoGDIα, increased and decreased, respectively, the numbers of invaded cells in MCF7 compared with control (c). The numbers of invaded cells in MDA-MB-231 cells treated with RhoGDIα siRNA (d) was increased in comparison to the upregulation (e) and control (f). (B) The mean numbers/per field of traversed cells onto the lower surface of porous membrane of transwell chambers after downregulation and upregulation of RhoGDIα in MCF7 and MDA-MB-231 cells with or without E2. *indicates a significant difference compared with the corresponding control.