Figure 1. Substrate-attached materials on laminin-511. (A) A549 cells were cultured on laminin-511-coated dishes for 2h30min. Cells were then treated with EGTA for 15 min and fixed. Scanning electron micrographs were obtained as described in Materials and Methods. Arrows indicate SAMs. (B) SAMs were prepared after detaching the cells by treatment with EGTA as described in Materials and Methods, following which they were separated by SDS-PAGE under reducing conditions and silver-stained. Lysates were also prepared from detached cells (CELL) and analyzed by SDS-PAGE. The positions of molecular weight markers are shown on the left. Arrows indicate laminin-511, which was used to coat dishes.