Figure 2. Detection of tetraspanins and their associated proteins in SAMs. (A) SAMs were prepared following the treatment of A549 cells cultured on laminin-511 with EGTA as described in Materials and Methods. Lysates (CELL) were also prepared from the cells detached by the EGTA treatment. SAMs and lysates were separated by SDS-PAGE, and subsequently immunoblotted with the antibodies indicated on the left of the blots. Data are representative of three separate experiments. The relative intensities of bands detected in SAMs to those in cell lysates are shown on the right. Values represent means ± SD from three independent experiments. (B) A549 cells cultured on laminin-511 were treated with EGTA. SAMs remaining on the substrates were immunostained with anti-CD9, anti-CD81, and anti-CD151 antibodies as described in Materials and Methods. Bar represents 10 μm.