Figure 3. Defects in Bergmann glial fibers morphology and Purkinje cell position. (A) Staining with anti-GFAP antibody on sagittal sections from P10, P17, and P21 control and α6fl/fl;nestin-Cre cerebellum to reveal Bergmann glial fibers. In the control samples the glial processes normally extended through the ML and branched to the basement membrane. In contrast, the glial fibers lacking α6 integrin appeared irregular with abnormal extensions. Glial fiber morphology is shown in each panel in high magnification views of the corresponding boxed area. The white arrows indicate the glial fibers. Scale bars, 100 μm; 20 μm. (B) P21 sagittal cerebellar sections stained with an anti-calbindin antibody to visualize Purkinje cells. In the control mice, Purkinje cells were aligned along the PCL, whereas in the α6fl/fl;nestin-Cre mice, some Purkinje cell bodies were ectopically localized in the ML. The asterisks indicate the mispositioned Purkinje cell in the mutant cerebellum. Scale bar, 50 μm. EGL, external granular layer; ML, molecular layer; PCL, Purkinje cellular layer; IGL, internal granular layer.