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. 2013 May 2;3:10. doi: 10.1186/2044-5040-3-10

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Copyright © 2013 Gross and Rotwein; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Defining myoblast population kinetics by live cell imaging. Individual EGFP-expressing myoblasts were studied as in Figure 2 using manual counting. Differentiation medium (DM) was added ± IGF-I (R3-IGF-I [2 nM]), as indicated. (A) Effect of IGF-I on total cell number. (B) Effect of IGF-I on the frequency of cell division (DM 234 divisions; IGF-I 344 divisions). (C) Effect of IGF-I on myoblast death (DM 208 deaths; IGF-I 154 deaths). For A to C, blue depicts control cells and red, myoblasts incubated with IGF-I. For B and C, the number of cells exhibiting a specific trait at a given time is plotted on the y-axis.