Adhesion of oral streptococci on tooth surfaces of mice. (A) Adhesion of S. mutans to the tooth surfaces of NOD, B10.D2, N5 (NOD.B10.D2), and C57BL/6 female mice at 4 months of age at different time points. CFU were determined in Mitis-Salivarius agar plates containing 0.02 M bacitracin after 48 h of anaerobic incubation at 37°C. (B) Adhesion of S. mutans for NOD, B10.D2 BC1, BC4, BC2, BC8, N5 (NOD.B10.D2), and N9 (NOD.B10.D2) female mice at 8 months of age 90 min after inoculation. CFU were determined in Mitis-Salivarius agar plates containing 0.02 M bacitracin after 48 h of anaerobic incubation at 37°C. (C) Adhesion of S. mutans, S. sobrinus, S. sanguis, S. mitis, and S. salivarius for N5 (NOD.B10.D2) female mice at 8 months of age 90 min after inoculation. CFU were determined in Mitis-Salivarius agar plates after 48 h of anaerobic incubation at 37°C. (D) Adhesion of S. mutans for NOD, B10.D2, N5 (NOD.B10.D2), and N9 (NOD.B10.D2) female mice at 8 months of age 24 h after inoculation. Data were obtained from three independent experiments with 15 mice in each strain, and values are expressed as the means ± SDs of the results. *, P < 0.05.