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. 2013 Jun 14;62(7):2416–2428. doi: 10.2337/db12-1380

FIG. 2.

FIG. 2.

Munc18b gain-of-function mutant potentiates GSIS. A: AdMunc18b mutant (-WT, -KR, and -E59K) transduction of rat islets did not influence the expression of SNARE or other SM proteins. Shown are representative of three independent experiments; analysis of Munc18b proteins expression shown in bottom panel. B: Confocal imaging showing AdMunc18b mutants did not influence Syn-2 or Syn-3 targeting to insulin SGs in rat β-cells. Shown are representative of four independent experiments. Scale bars, 10 μm. C and D: Islet perifusion assays showing AdMunc18b mutant–transduced rat islets influencing GSIS (C) and 10 nmol/L GLP-1 plus 150 µmol/L IBMX–potentiated GSIS (D). Data shown are means ± SEM from three to four sets of experiments, with each experiment performed simultaneously in all of the four conditions. Bottom panels show quantification of AUC analysis of first-phase (encompassing 11–22 min) and second-phase (encompassing 22–40 min) GSIS. *P < 0.05, **P < 0.01, ***P < 0.001 by one-way ANOVA. E: Effects of AdMunc18b mutants on GSIS and 10 nmol/L GLP-1 plus 150 µmol/L IBMX-potentiated GSIS in INS-1 cells. Results shown are means ± SEM from three independent experiments performed in duplicates or triplicates (n = 6–8). *P < 0.05 by ANOVA and Scheffé tests.