FIG. 4.

The late G1/S cyclins/Cdks. A: Each of the cyclin A, cyclin E, and cdk1 and -2 members of late G1/S complexes is present in the cytoplasmic, but not nuclear, compartment. B: Adenoviral overexpression enhances the abundance of the late G1/S molecules and, in some cases, e.g., cyclin E and cdk2, leads to their nuclear appearance. Each panel is representative of 3–5 experiments on different human islet preparations. Labeling with no primary antibody or with primary antibody plus blocking peptide is shown as negative controls. Ins, insulin.