FIG. 5.

Ceramide induces food intake in CPT1C KO mice. WT and CPT1C KO mice were treated with ICV vehicle (white bars) or 2.5 μg ICV ceramide C6:0 (black bars) at the beginning of the light cycle. Food intake (A) was measured 3 h after the injection. AgRP (B) and NPY (C) mRNA levels in MBH were measured by real-time PCR in samples obtained 3 h after the treatment. *P < 0.05, **P < 0.01 vs. vehicle-treated mice. CER, ceramide.