FIG. 5.

miR-93 targets Glut4 in 3T3-L1 adipocytes. Differentiated 3T3-L1 adipocytes were transfected with miR-93 plasmid (2 μg) or empty vector. 3T3-L1 adipocytes with induced miR-93 (A) have decreased Glut4 gene expression (B). C and D: Comparison of Glut4 (red) in 3T3-L1 adipocytes transfected with an GFP-miR-93 vector or empty vector (green) and expression of Glut4 (red); scale bars are 50 μm. E: Luciferase Glut4 3′UTR plasmid was cotransfected with either miR-93 or empty vector. Results were obtained from three independent experiments. F and G: miR-93 inhibition increases Glut4 gene expression in 3T3-L1 adipocytes.