Fig. 2.

Sensitivities and specificities of Acanthamoeba LAMP assays. LAMP assays were performed using serial dilutions of (A) plasmid DNA containing Acanthamoeba castellanii 18S rDNA (10, 10², 10³, or 10⁴ copies per reaction) and (B) genomic DNA (100, 10, or 1 pg). Plasmid containing no insert was used as a control. LAMP products were visualized by (C) gel electrophoresis and using (D) the Loopamp® fluorescent detection reagent (FD). Lanes M1 and M2, 1-kb and 100-bp molecular weight markers, respectively; lane 1, Acanthamoeba astronyxis; lane 2, Acanthamoeba triangularis; lane 3, Acanthamoeba rhysodes; lane 4, Acanthamoeba castellanii; lane 5, Acanthamoeba lugdunensis; lane 6, Acanthamoeba polyphaga; lane 7, Acanthamoeba quina; lane 8, Acanthamoeba griffini; lane 9, Acanthamoeba hatchetti; lane 10, Acanthamoeba culbertsoni; lane 11, Acanthamoeba healyi; lane 12, Aspergillus fumigatus; lane 13, Fusarium solani; lane 14, Candida albicans; lane 15, Entamoeba histolytica; lane 16, Giardia lamblia; lane 17, Escherichia coli; lane 18, distilled water; and lane 18, NcoI digestion of the LAMP product of 18S rDNA.