Fig. 4.

Independent segregation of RMCE acceptor loci in F2 progeny from pig #2772. Pig #2772 was bred with a wt pig to create 27 piglets with various RMCE acceptor loci. a The acceptor loci were revealed by Southern blot using the same condition as in Fig. 1c. The F2 pigs number listed beneath each lane. DNA ladder marked M. b The RMCE acceptor loci integration site were confirmed by PCR using a GFP primer and a genomic primer specific for each integration site. c The table summarizes the RMCE acceptor loci present and lists the gender of each F2 pig