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. 2012 Apr 1;71(7):618–626. doi: 10.1016/j.biopsych.2011.11.030

Figure 5.

Figure 5

Comparison of BE5.2(T) and BE5.2(C) interaction with brain derived neurotrophic factor promoter 4 (BP4) in potassium chloride (KCl) depolarized hippocampal, cortical, and amygdala cultures. Primary hippocampal (A, D), cortical (B, E), and amygdala (C, F) cultures were co-transfected with either BP4-BE5.2(T) or BP4-BE5.2(C) with renilla plasmid. Cells were untreated (A–C) or treated (D–I) with 60 mmol/L KCl for 24 hours before harvest for dual luciferase assay. Relative light units were calculated by normalizing luciferase signal to renilla signal. Fold induction was calculated by dividing relative light units of treated culture by relative light units of vehicle-treated cultures. n ≥ 3, **p < .01, *p < .05. n.s., not significant.