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. 2012 Apr 1;71(7):618–626. doi: 10.1016/j.biopsych.2011.11.030

Figure 7.

Figure 7

Combined treatment of phorbol-12-myristate-13-acetate (PMA) and forskolin increased luciferase expression by brain derived neurotrophic factor promoter 4 (BP4) BE5.2 (T) and BP4 BE5.2 (C) in cortical cultures. Primary cortical cultures were co-transfected with either (A) BP4 or (B, C) BP4 BE5.2(T) and BP4 BE5.2(C). Cells were treated with 150 nmol/L PMA and 25 μmol/L forskolin or dimethyl sulfoxide (DMSO) as vehicle treatment for 24 hours before harvest for dual luciferase assay. Relative light units were calculated by normalizing luciferase signal to renilla signal. Fold induction was calculated by dividing relative light units of treated culture by relative light units of vehicle-treated cultures. n ≥ 3, **p < .01, *p < .05, and #two-way analysis of variance p > .05 but t test p < .05.