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ECM protein patterning protocol. An initiator molecule or macromolecule is micro-contact printed on a substrate (gold-coated glass or glass), followed by POEGMA brush growth. At this stage, substrates can be stored in ambient conditions for several weeks without impairing their properties. For ECM protein deposition, a substrate is immersed in a solution of the desired protein, washed and used for cell seeding or immuno-staining.
