TABLE 1.
Assessment of the neutralization assay using flow cytometry: results of controls in three different experiments
| Serotype and culture component(s) | % of A549 transduction in exp no.:
|
Mean | SD | ||
|---|---|---|---|---|---|
| 1 | 2 | 3 | |||
| AD5 | |||||
| A549 only | 6.82 | 6.71 | 5.44 | 6.32 | 0.77 |
| A549 + AD5EGFP | 94.7 | 91.14 | 91.70 | 92.51 | 1.91 |
| A549 + AD5EGFP + positive serum | 2.82 | 8.31 | 6.94 | 6.02 | 2.86 |
| A549 + A5EGFP + negative serum | 81.19 | 78.90 | 88.74 | 82.94 | 5.15 |
| AD35 | |||||
| A549 only | 6.64 | 6.62 | 1.92 | 5.06 | 2.72 |
| A549 + AD35EYFP | 95.54 | 97.10 | 97.67 | 96.77 | 1.10 |
| A549 + AD35EYFP + positive serum | 1.26 | 8.17 | 2.36 | 3.93 | 3.03 |
| A549 + AD35EYFP + negative serum | 85.29 | 83.24 | 83.19 | 83.90 | 0.98 |