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Detection of active RNAP II. (A–E) Immunofluorescence for RNAP II (green), cytokeratin 7 (red) and DAPI (blue). Examples of RNAP II-immunoreactive STB nuclei are illustrated by arrows. (F) Negative control (omission of primary antibodies). Gestational ages (weeks + days) are given at the top right of the images. Scale bar = 20 μm for all images.
