Table 2.
mRNA levels of selected genes involved in heart development in P19CL6 cell lines with and without induction to generate a cardiomyocyte phenotype1.
| Gene | Cell line2 | mRNA level: noninduced cells (U) | mRNA level: induced cells (I) | I/U3
|
|---|---|---|---|---|
| SRF | Mock clones #1, 2, 3 | 1.08 ± 0.11 | 0.46 ± 0.08 | 0.43 ± 0.01 |
| mRhoAN19 clone #1 | 0.60 ± 0.12 | 1.68 ± 0.11* | 2.75 ± 0.06* | |
| Cardiac α-actin | Mock clones #1, 2, 3 | 0.97 ± 0.04 | 0.63 ± 0.17 | 0.65 ± 0.02 |
| mRhoAN19 clone #1 | 1.14 ± 0.14 | 2.93 ± 0.35* | 2.57 ± 0.04* | |
| GATA4 | Mock clones #1, 2, 3 | 1.00 ± 0.48 | 0.35 ± 0.05 | 0.35 ± 0.04 |
| mRhoAN19 clone #1 | 0.70 ± 0.12 | 2.41 ± 0.38* | 3.44 ± 0.09* |
1Data represent the results of 3–6 independent measurements.
2Values for mock transfected (Mock) clones #1, 2, 3 represent combined results for these three cell lines.
3I/U is the ratio of mRNA levels in induced (I) and uninduced (U) cells.
*P < 0.05, compared to mock transfected cell lines.