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Effects of H/R on cell viability and LDH release in H9c2 cardiomyocytes. H9c2 cardiomyocytes were exposed to 6 h of hypoxia, followed by 24 h of reoxygenation. Then, cell viability was detected at 0, 2, 4, 8, 12, and 24 h after reoxygenation using CCK8 assay (a). Cell death was also measured by LDH assay kit (b). The results are represented as means ± SD from three independent experiments. *P < 0.05 versus control group.
