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. 2012 Dec 13;68(8):877–891. doi: 10.1093/gerona/gls242

Figure 1.

Figure 1.

(A) Quantitative real-time reverse transcription-polymerase chain reaction data showing age-related downregulation of mRNA expression of Dicer in microdissected small branches of the middle cerebral arteries in F344 × BN rats. Data are mean ± SEM (n = 5–7). *p< .05 vs 3-month-old controls. (B) Flow cytometric analysis of endothelial markers in primary cerebromicrovascular endothelial cells (CMVECs) derived from young (3 months old) and aged (24 months old) F344 × BN rats. Analysis of anti-CD31, anti-CD146, anti-EpoR, and anti-VEGFR2 reactivity (filled areas) shows the high purity of endothelial cell cultures. Appropriate isotype controls (blank areas) are also shown. (C) Original Western blots showing protein expression of Dicer in CMVECs isolated from 3- and 24-month-old F344 × BN rats. β-Actin was used for normalization purposes. Bar graphs (Panel D) are summary densitometric values. Data are mean ± SEM. *p < .05 vs 3-month-old control.