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Number of unique 96-bp sequences (y axis, log scale) in C. elegans PstI RAD-Seq library (14 PCR cycles, Replicate 1) with read depths from 0 to 1000 reads (x-axis). An additional long tail of high-count (>1000) unique, repeat-derived sequences is not shown. The red line shows a candidate threshold separating sequencing error from genuine alleles; the green curve shows a theoretical expectation of coverage for homozygous RAD loci.
