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. 2013 Jan 9;11(5):535–545. doi: 10.1111/pbi.12041

Figure 1.

Figure 1

Schematic representation of the constructs used in tobacco BY-2 cell transformation. NPT II, neomycin phosphotransferase II gene under the control of the nopaline synthase promoter and terminator; cauliflower mosaic virus (CaMV)-35S, double-enhanced cauliflower mosaic virus 35S promoter. NSP, human IL-10 native signal peptide (54 bp); IL-10, human IL-10 coding sequence (480 bp); Thr, Thombin protease recognition sequence (27 bp); HIS, six histidine tag; KDEL, ER-retrieval tetrapeptide; TNOS, nopaline synthase terminator; tobacco etch virus (TEV), tobacco etch virus protease recognition site (21 bp); green fluorescent protein (GFP), smGFP coding sequence (714 bp); TE, translational enhancer from the tCUP promoter (88 bp) (Wu et al., 2001); Pr1b, signal peptide from the tobacco pathogenesis-related 1b gene (90 bp); ELP, elastin-like polypeptide (420 bp); SII, StrepII purification tag (24 bp); RB, LB, Agrobacterium tumefaciens Ti plasmid right border and left border. Constructs are not drawn to scale.