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. 2013 Jan 9;11(5):535–545. doi: 10.1111/pbi.12041

Figure 4.

Figure 4

Purification and biological activity of IL-10-ELP. (a) Stained gel of purified IL-10-ELP protein. Lane 1, protein marker; lanes 2–5, increasing volumes of purified IL-10-ELP product; lanes 6–10, increasing amounts of purified, commercial BSA standard. (b) Biological activity analysis of IL-10-ELP. The cell culture supernatant of 4 × 105 PU5-1.8 cells (▲), lipopolysaccharide(LPS)-stimulated cells (◯), LPS-stimulated cells treated with commercial rhIL-10 (◊), IL-10-ELP (•), IL-10 moiety of IL-10-ELP after cleavage of fusion and removal of ELP (▪). An equal volume of sample purified from wild-type BY-2 cells (▽) was used as a negative control.