Table S1.
PCR and competitive PCR primer sequences.
| Primer # | Primer name | Sequence (5′ to 3′) | Notes |
|---|---|---|---|
| 1 | CEBPG rs2772 R common | AGA TCT AAC AGC TGC AGA ATG G | Reverse primer for pre-amplification of both A and C allele at rs2772. |
| 2 | CEBPG rs2772 R-A1 | AGA TCT AAC AGC TGC AGA ATG GA | Matches A allele at rs2772. Did not meet allele-specificity criteria. |
| 3 | CEBPG rs2772 R-A2 | AGA TCT AAC AGC TGC AGA ATG AA | Matches A allele at rs2772 with 3′ pen-ultimate mismatch. Pen-ultimate mismatch decreased overall PCR efficiency, however it did achieve allele-specificity criteria. |
| 4 | CEBPG rs2772 R-A3 | AGA TCT AAC AGC TGC AGA ATG TA | Matches A allele at rs2772 with 3′ pen-ultimate mismatch. Good PCR efficiency and met allele-specificity criteria. Used with CEBPG rs2772 F1 primer for ASE of CEBPG. |
| 5 | CEBPG rs2772 R-A4 | AGA TCT AAC AGC TGC AGA ATG CA | Matches A allele at rs2772 with 3′ pen-ultimate mismatch. Pen-ultimate mismatch decreased overall PCR efficiency, however it did achieve allele-specificity criteria. Artifact peaks present. |
| 6 | CEBPG rs2772 R-C1 | AGA TCT AAC AGC TGC AGA ATG GC | Matches C allele at rs2772. Good PCR efficiency and met allele-specificity criteria. |
| 7 | CEBPG rs2772 R-C2 | AGA TCT AAC AGC TGC AGA ATG AC | Matches C allele at rs2772 with 3′ pen-ultimate mismatch. Good PCR efficiency and met allele-specificity criteria. |
| 8 | CEBPG rs2772 R-C3 | AGA TCT AAC AGC TGC AGA ATG TC | Matches C allele at rs2772 with 3′ pen-ultimate mismatch. Good PCR efficiency and met allele-specificity criteria. Used with CEBPG rs2772 F1 primer for ASE of CEBPG in order to maintain consistency of 3′ pen-ultimate mismatch between both allele-specific primers. |
| 9 | CEBPG rs2772 R-C4 | AGA TCT AAC AGC TGC AGA ATG CC | Matches C allele at rs2772 with 3′ pen-ultimate mismatch. Pen-ultimate mismatch decreased overall PCR efficiency, however it did achieve allele-specificity criteria. Artifact peaks present. |
| 10 | CEBPG rs2772 F1 | TTT CCC AGT CCC CAT TAG AGG | Forward primer used for general pre- and allele-specific amplification. |
| 11 | CEBPG rs2772 IS A allele | AGA TCT AAC AGC TGC AGA ATG GAA AAC AAA ACA ATT CTC CTA AGG C | Primer coupled with CEBPG rs2772 F1 to create A allele competitive template. |
| 12 | CEBPG rs2772 IS C allele | AGA TCT AAC AGC TGC AGA ATG GCT TCT CCT AAG GCA TCA GA | Primer coupled with CEBPG rs2772 F1 to create C allele competitive template. |