Figure 1. Over-expressed ACTN4 co-immunoprecipitates with RelA/p65 in the cytoplasm of HEK293T cells but does not mediate RelA/p65 nuclear translocation.

(A) RelA/p65 was immunoprecipitated from cytoplasm protein extracts of HEK293T cells with RelA/p65-specific antibody. Bound proteins were separated by SDS-PAGE followed by immunoblotting with anti-MYC and anti-RelA/p65 antibodies. No RelA/p65 specific signal was detected after incubation of cytoplasm extracts with empty Protein G Sepharose beads (PG lane). (B) Western blot analysis of cytoplasm and nuclear protein extracts using MYC-, RelA/p65- and hnRNPA2/B1-specific antibodies. hnRNPA2/B1 was used as a loading control.