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. Author manuscript; available in PMC: 2013 Jul 16.
Published in final edited form as: Nature. 2012 Aug 16;488(7411):337–342. doi: 10.1038/nature11331

Figure 2. Homozygous deletion of the 1p36 locus in GBM results in loss of ENO1 expression in primary tumors and cell lines.

Figure 2

a, TCGA Affymetrix aCGH data show four primary GBMs with log2 copy number < −1, indicating homozygous deletion of the 1p36 locus. b, DNA copy number correlates with mRNA expression; expression is highest in tumors with n = 2 copies (WT) and lowest in tumors with n = 0 copies (null) of ENO1. c, The D423-MG cell line was identified as homozygously deleted by SNP arrays from the Wellcome Trust Sanger Institute data set. d, The complete absence of enolase 1 protein in D423-MG and Gli56 cells was confirmed by western blotting. e, shRNA knock-down of ENO2 in D423-MG ENO1 null cells ablated intracranial tumorigenesis in vivo (n=4 mice per group).