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. Author manuscript; available in PMC: 2013 Jul 16.
Published in final edited form as: Cell Rep. 2012 Nov 29;2(6):1762–1773. doi: 10.1016/j.celrep.2012.10.026

Figure 3. NADPH Oxidase Mediates Neutrophil-Dependent Conidial Killing in the Lung.

Figure 3

BM chimeric mice (1:1 mix of CD45.1+p47phox(+/+) and CD45.2+p47phox(−/−) BM cells → irradiated CD45.1+CD45.2+ recipients) were infected with 3 × 107 FLARE conidia.

(A) Representative plots of p47phox(+/+) and p47phox(−/−) neutrophils analyzed for dsRed and AF633 fluorescence show the frequencies of neutrophils that contain live (red gate) or killed (blue gate) conidia 36 hr p.i.

(B and C) Scatterplots from one (36 hr p.i.) or two (12 hr p.i.) experiments show the average frequency (±SEM) of (B) lung neutrophil conidial uptake (R1 + R2) and (C) lung neutrophil conidial viability (R1/(R1 + R2)) by p47phox(+/+) (circles) or p47phox(−/−) (diamonds) neutrophils. *p < 0.05 (paired t test).