Figure 5.

Induction of TRP-2-specific CD8⁺ T cell responses in ret transgenic mice. Mice with (ret tu) or without macroscopic tumors (ret) or non-transgenic littermates (wt) were immunized with the peptide derived from TRP-2. Frequencies and functional activities of TRP-2-specific spleen CD8⁺ T cells were analyzed ex vivo by tetramer staining (a) and IFN-γ ELISPOT assay (b–d). Control animals were injected with PBS. (a) Representative dot plots for tetramer staining are shown; (b–d) Cumulative data (mean ± SEM; 6–8 mice per group) for IFN-γ secreting CD8⁺ T cells are shown as numbers of IFN-γ spots among 1 × 10⁶ splenocytes.