. Author manuscript; available in PMC: 2014 Jun 25.

Published in final edited form as: Biochemistry. 2013 Jun 13;52(25):4331–4342. doi: 10.1021/bi400182y

Table 1.

Predicted and observed distribution of intermediates in RFQ experiments

t = 0 s		t = 320 ms		t = 3 s

Prediction (At start)	Observation (Mössbauer)	Prediction (Kinetics)	Observation (Mössbauer)	Prediction (Kinetics)	Observation (Mössbauer)
100 % H^red	95 % diferrous	55 % H_sl^red	86 % diferrous	50 % H_sl^red	58 % diferrous
		33 % P*	86 % diferrous	10 % P*	58 % diferrous

		3.4 % P	3 % P	4 % P	7 % P
		3.6 % (Q + H^ox)	5 % H^ox	22 % Q	23 % Q
				2 % H^ox	7 % H^ox+ H_sl^ox
				6 % H_sl^ox

	5 % unidentified^a	5 % unidentitied^b	5 % unidentified^a	5 % unidentified^b	5 % unidentified^a

In all samples, approximately 5 % of the Mössbauer absorption could not be positively identified as it competes with the noise. This absorption is probably associated with diferric species (not a single one). It is certainly not originating from ferrous MMOH and may derive in part from unreduced enzyme.

Assumed from the Mössbauer spectrum of the t = 0 sample.