Table 1.
DNA primers were employed for reverse transcription-polymerase chain reaction (RT-PCR) amplification of grapevine viruses and for control mRNA sequenc
| Gene | Product size (bp) | Location | Sequences 5′-3′ | Target Primer |
|---|---|---|---|---|
| GFLV | f-TGCTGGATATCGTGACCCTGT | 5506-5527 | 118 | RNA dependent |
| r-AAGGTATGCCTGCTTCAGTGG | 5602-5623 | RNA polymerase | ||
| GFkV | f-TGACCAGCCTGCTGTCTCTA | 6453-6472 | 179 | Coat protein |
| r-TGGACAGGGAGGTGTAGGAG | 6612-6631 | |||
| GVA | f-AGGTAGATATAGTAGGACCTA | 6591-6612 | 272 | Coat protein |
| r-TCGAACATAACCTGTGGCTC | 6843-6862 | |||
| GLRaV-3 | f-ACGTTAAGGACGGGACACAGG | 13383-13404 | 336 | Coat protein |
| r-TGCGGCATTAATCTTCATTG | 13699-13718 | |||